Proline

L Proline is one of the two dominant amino acids in collagen (alongside hydroxyproline), making up approximately 10 to 15% of collagen's total amino acid content. Each capsule provides 340mg of L proline in a vegetarian capsule. Proline is classified as a conditionally essential amino acid, meaning the body can synthesise it under normal conditions but demand can exceed supply during wound healing, injury recovery, high physical stress or ageing.

In the body, proline is hydroxylated (with vitamin C as a cofactor) to form hydroxyproline, which is critical for collagen stability. Without sufficient proline and vitamin C, collagen structure weakens, affecting skin elasticity, joint cartilage integrity, blood vessel walls, tendon strength and wound healing. Supplementing proline directly provides the raw material for collagen synthesis without requiring the body to convert other amino acids. This is particularly relevant for those following plant based diets, where dietary proline intake is naturally lower than in diets containing animal proteins and bone broth.

Take 2 capsules twice per day with meals.

The four capsule daily dose provides 1360mg of L proline. Taking with meals supports absorption and provides the proline alongside other dietary amino acids for balanced protein utilisation.

For collagen support, pair with vitamin C (required for the hydroxylation of proline into hydroxyproline) and consider combining with glycine (the other major collagen amino acid). For joint health, adding hyaluronic acid and N acetyl glucosamine provides a comprehensive cartilage support protocol. Allow 8 to 12 weeks of consistent use to assess improvements in skin, joint or connective tissue health.